KLONOWANIE DNA PDF

Blog. 18 December Prezi Awards The best presentations have arrived. 5 December Do this, not that: Keynote speech. 28 November Wady i Zalety Klonowania Idea 1. Idea 2. Rozmnażanie bezpłciowe – naturalne klonowanie. Klonowanie zachodzi przez: Klonowanie DNA. KLONOWANIE Klony DNA służą do: Co to jest klonowanie? Klonowanie – tworzenie genetycznej kopii fragmentu DNA, komórki lub organizmu.

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Let’s take a closer look at each step.

In some cases, it doesn’t. Not all colonies will necessarily contain the right plasmid.

The restriction enzymes are just in mass cutting these things. In other cases, bacteria may be used as protein factories.

Selekcja i transformacja u bakterii

DNA ligase, to connect the backbones right over here. So we use restriction enzymes. In some genetic disorders, patients lack the functional form of a particular gene. And so this is a pretty cool thing.

So that might be a restriction enzyme right over there and then you might use another restriction enzyme that identifies with the sequence at the other side that we wanna cut. In some cases, we need lots of DNA copies to conduct experiments or build new plasmids. Gene cloning – advanced. In some cases, plasmids are directly used for practical purposes. In the final step, after all the non-target proteins have been washed away, the target proteins are released from the antibodies in the column, and the pure protein is collected for use.

Plasmids used in cloning contain an antibiotic resistance gene. What we want to get is:. The promoter “points” towards the right, meaning that it will drive transcription of the DNA sequence that lies to the right.

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A recombinant plasmid where the target gene is inserted after the promoter, pointing in the forward direction oriented so that it’s transcribed to make an mRNA that specified the desired protein. Suppose that we identify a colony with a “good” plasmid. Campbell biology edycja 10, strony And this is amazing because obviously DNA, this isn’t stuff that we can, you know, manipulate with our hands the way that we would copy and paste things with tape.

So that is DNA ligase, which you can think of it as helping to do, helping to do the pasting. Antibiotic, and so this one will survive ’cause it has that resistance. Transfer of plasmid DNA into bacteria. Expression of the gene leads to production of mRNA, which is translated into protein.

Kosmid – Wikipedia, wolna encyklopedia

Steps of DNA cloning. So then so this is where we cut, let me write this, we cut out the gene and then we wanna paste it then we wanna paste it into a plasmid. However, if we want to express the gene in bacteria to make a protein, the gene must point in the right direction relative to the promoter jlonowanie, or control sequence that drives gene expression.

Protein production in bacteria. Cutting and pasting DNA. For example, DNA cloning was used to build plasmids containing a normal version of the gene that’s nonfunctional in cystic fibrosis.

Serial Cloner – Download

Overview of DNA cloning. In the final step, the protein of interest is released from the column and collected for use. DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene. Insert the plasmid into bacteria. So let me, we’re pasting it into the plasmid. Bacteria without a plasmid die.

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Each bacterium with a plasmid gives rise to a cluster of klonoaanie, plasmid-containing bacteria called a colony.

Ddna like restriction enzyme digestion and PCR are commonly used to check the plasmids. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony. Cells that have produced protein are burst klonowane lysedreleasing the protein and the other cell contents. You might have a little bit left over on either side but essentially you have cut out the gene. You have a vial and it has a solution in it with a bunch of E.

Przegląd: Klonowanie DNA

In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid. A bunch of E. And as it reproduces it also is reproducing the plasmids and because it has this antibiotic resistance it is going to grow on this nutrient antibiotic mixture and the other bacteria that did not take up the plasmids are not going to grow.

So let me draw that.

Selekcja i transformacja u bakterii. Each surviving bacterium will give rise to a small, dot-like group, or colonyof identical bacteria that all carry the same plasmid.