Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 2 talking about this · 1 was here. Laboratorio de. Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 1 was here. Laboratorio de Ecotoxicología Acuática (LEA). Laboratorio de Ecotoxicología Acuática in Junín De Los Andes – Contact details, Address Map, Photos, offers, Real time Reviews and Ratings.
|Published (Last):||16 August 2004|
|PDF File Size:||2.83 Mb|
|ePub File Size:||2.6 Mb|
|Price:||Free* [*Free Regsitration Required]|
The maximum induction of CYP1A transcript expression has been reported to start eight to sixteen hours after exposure Minura et al.
The study was conducted in two m-long reaches: Evolution of pharmacologic specificity in the pregnane X receptor. B Oil spill sources running towards the stream. This explains the similar response in ecotoxicologi CYP1A transcript levels at 24 and 96 h during our experiments, without dose-dependent effects.
Bands were analyzed densitometrically using Image J software.
ecotoxicopogia A Oil remains afloat on the surface of stream water. Considering the public concern ecohoxicologia adverse health effects of persistent oil pollution on highly valued aquatic ecosystems, particularly on water and edible fish, it is necessary to develop tools to assess the impact of this oil spill.
The WAF was prepared according to Singer et al. We found induced CYP3A27 transcript levels only in liver of impacted wild trout, suggesting an induction at longer time than four days.
In vitro effects of endosulfan-based insecticides on mammalian sperm.
TPH concentration in sediments was 0. The stream bottom is composed of boulder cobble substrates and the stream acuztica has alternated riffle-pool habitats. Predictors of relative fish biomass in lakes and reservoirs of Argentina. Induction patterns of new CYP1 genes in environmentally exposed rainbow trout. Thus, anomalous expression of both CYP2 could impact on steroid and fatty acid homeostasis in rainbow trout e.
Seven CYP families have been reported for the rainbow trout Uno et al.
Ecotoxicologia acuatica – Jaime Alberto Palacio Baena – Google Books
acuatics CYP1A mRNA expression is induced by minuscule amounts of diverse aromatic compounds through the aryl hydrocarbon receptor AhR signaling pathway, being a sensitive biomarker of exposure to aromatic hydrocarbon contaminants Uno et al. In order to test if orally supplied Euglena sp.
Samples were diluted with ultra-pure water to adjust to a concentration of 2.
These clear and consistent changes reaffirm CYP1A transcript expression as a good semiquantitative biomarker for crude oil exposure.
Letters denote significant differences among treatments within each time, 24 h lowercase letters and 96 h capital letters from Bonferroni test Table II. The study of mRNA expression patterns of multiple CYP genes in rainbow trout gills and liver would be extended in acuatics to evaluate their use for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems. The climate is cold-temperate, with a mean annual temperature of 8.
Rainbow ecotoxicologiz cytochrome Ps: The results obtained in wild rainbow trout could be particularly useful to evaluate other freshwater pollution, since the TPH concentration varied along the stream reach and rainbow trout was not exposed to a constant concentration of the pollution for ecotoxicolobia hours, unlike the experiments. Triplicate samples were collected at the upstream reach —, — and — mat the scuatica spill zero metersat the downstream reach 50, and m and further downstreamand m.
Evaluating potential effects of exotic freshwater fish from incomplete species presence-absence data. A bp ladder Winkler was used as the molecular mass marker. Antioxidant catalase and detoxifying gluthatione S-transferase defenses are generally lower in gills and digestive gland of EG mussels than in SV ones.
This, along with the fact that its metabolic pathways related to oil contaminants detoxification are relatively well known, makes this species a good bioindicator Moore et al. Our results show that the total hemocyte number decreases while pollution load increases along the river course for both, EG and SV mussels.
Data was complemented with laboratory exposure to water accommodated fraction WAF from the oil spill. Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure. Cytochrome P CYP gene expressions were studied by semi-quantitative polymerase chain reaction PCR to assess oil pollution using liver and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream Bariloche, Argentinaup and downstream of a discharge of petroleum spill from an unproductive wellbore.
Departamento de Química Biológica – FCEN, UBA
Consequently, since several CYPs could be influenced by exposure to polycyclic aromatic hydrocarbons, oil contamination could affect biosynthesis and metabolism of many important endogenous constituents of rainbow trout. Since direct measurement of tissue hydrocarbon concentrations generally does not provide a useful indicator of petroleum exposure in fish and could be expensive Collier et al. Excretion of aromatic hydrocarbons and their metabolites by freshwater and seawater Dolly Varden char.
The annealing temperatures and primer pairs used during the duplex PCR for each gene are shown in Table I. We focused on gill and liver tissues because the gills are the primary route of hydrocarbon uptake from water, and the liver is the main organ for metabolism of toxic oil constituents Thomas and Rice Expression and inducibility of aryl hydrocarbon receptor pathway genes in wild-caught killifish Fundulus heteroclitus with different contaminant-exposure histories.
CYPs transcriptional expression in wild rainbow trout. In contrast, more extended time and high PAH concentration e. Both compound classes caused paralysis and immune impairment. Informe, Buenos Aires, Argentina, 64 pp. Lysosomal membrane stability is higher in EG mussels than in SV ones for all conditions, although it is decreased at polluted sites compared with that at C.